Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspr antibody [EPR7828] (ab133634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7828] to Caspr
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspr antibody [EPR7828]
    See all Caspr primary antibodies

  • Description

    Rabbit monoclonal [EPR7828] to Caspr

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Caspr aa 700-800 (extracellular). The exact sequence is proprietary.

  • Positive control

    • C6 cell lysate, Neuro2a cell lysate, U87 MG cell lysate, Fetal brain lysate, Fetal lung lysate, Human glioma tissue. ICC/IF: Neuro-2a cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.40 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7828

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CNTNAP1 (Caspr) knockout HAP1 whole cell lysate
    Lane 3 : MEF whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 156 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133634 observed at 175 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab133634 was shown to recognize Caspr in wild-type HAP1 cells as signal was lost at the expected MW in CNTNAP1 (Caspr) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CNTNAP1 (Caspr) knockout samples were subjected to SDS-PAGE. ab133634 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [EPR7828] (ab133634)

    Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Caspr with purified ab133634 at 1/100 (5.7 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)

    Immunohistochemical analysis of paraffin embedded Human glioma tissue labelling Caspr with ab133634 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Neuro2a cell lysate
    Lane 3 : U87 MG cell lysate
    Lane 4 : Fetal brain lysate
    Lane 5 : Fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 156 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Caspr antibody [EPR7828] (ab133634)
    OI-RD Scanning - Anti-Caspr antibody [EPR7828] (ab133634)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Caspr antibody [EPR7828] (ab133634)
    Anti-Caspr antibody [EPR7828] (ab133634)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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