Anti-CNPase antibody (ab227218)
Key features and details
- Rabbit polyclonal to CNPase
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CNPase antibody
See all CNPase primary antibodies -
Description
Rabbit polyclonal to CNPase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human CNPase (internal sequence). The exact sequence is proprietary.
Database link: P09543 -
Positive control
- WB: HeLa cell extract; MOLT-4 whole cell lysate; Mouse and rat brain tissue extracts. IHC-P: U87 xenograft tissue. IHC-Fr: Adult mouse cerebellum and hippocampus tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-CNPase antibody (ab227218) at 1/500 dilution + CNPase Knockout HeLa cell extracts at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 45,48 kDa
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Frozen sections of adult mouse cerebellum tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution (red). Nuclear Counterstain: Fluoroshield with DAPI (blue).
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All lanes : Anti-CNPase antibody (ab227218) at 1/500 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : CNPase knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45,48 kDa10% SDS-PAGE gel.
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Paraffin-embedded U87 (human) xenograft tissue stained for CNPase using ab227218 at 1/500 dilution in immunohistochemical analysis.
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Frozen sections of adult mouse hippocampus tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: NeuN (red), stained by NeuN antibody at 1/500 dilution.
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Anti-CNPase antibody (ab227218) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 45,48 kDa10% SDS-PAGE gel.
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All lanes : Anti-CNPase antibody (ab227218) at 1/5000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 45,48 kDa10% SDS-PAGE gel.