Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [EFR163M] to Ephrin B2 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free
    See all Ephrin B2 primary antibodies

  • Description

    Mouse monoclonal [EFR163M] to Ephrin B2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Ephrin B2 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P52799

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human skin and tonsil tissue. ICC/IF: A431 and A549 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252795 is the carrier-free version of ab241556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241556).

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Ephrin B2 with ab241556 at 4.64 µg/ml Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)
    Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241556).

    Immunofluorescent analysis of 100% methanol-fixed A431 (human epidermoid carcinoma epithelial cell line) cells labeling Ephrin B22 with ab241556 at 9.28 µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on A431 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab241556 at 9.28 µg/ml, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241556).

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ephrin B2 with ab241556 at 4.64 µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on epithelial cells of human tonsil tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)
    Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab241556).

    Immunofluorescent analysis of 100% methanol-fixed A549 (human lung carcinoma epithelial cell line) cells labeling Ephrin B22 with ab241556 at 9.28 µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on A549 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab241556 at 9.28 µg/ml, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)
    Anti-Ephrin B2 antibody [EFR163M] - BSA and Azide free (ab252795)

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