Anti-Ephrin B2 antibody (ab131536)
Key features and details
- Rabbit polyclonal to Ephrin B2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Ephrin B2 antibody
See all Ephrin B2 primary antibodies -
Description
Rabbit polyclonal to Ephrin B2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatHumanIHC-P RatHumanWB RatHuman
-
Immunogen
Synthetic peptide corresponding to Human Ephrin B2 aa 328-332 conjugated to keyhole limpet haemocyanin.
Sequence:N-I-Y-Y-K
Database link: P52799 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody (ab131536)
ab131536 staining Ephrin B2 in formalin-fixed, paraffin embedded rat C6 cells. Samples were incubated with primary antibody at 1/75 dilution. A Goat anti-rabbit IgG H&L (1/50) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody (ab131536)Immunohistochemical analysis of rat kidney tissue sections at 1/100 dilution. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody (ab131536)Immunohistochemical analysis of mouse kidney tissue sections at 1/100. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
-
Western blot - Anti-Ephrin B2 antibody (ab131536)Anti-Ephrin B2 antibody (ab131536) at 1/500 dilution + HT29 cell extract
Predicted band size: 36 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody (ab131536)Immunohistochemical analysis of Human renal clear cell carcinoma tissue sections labeling Ephrin B2 with ab131536 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. the tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated Goat Anti-Rabbit antibody was used as the secondary.
-
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody (ab131536)ab131536 staining Ephrin B2 in formalin-fixed, paraffin embedded HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1/75 dilution. A Goat anti-rabbit IgG H&L (1/50 dilution) was used as the secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody (ab131536)Immunofluorescence analysis of methanol-fixed HeLa cells labelling Ephrin B2 with ab131536 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 antibody (ab131536)Immunohistochemical analysis of Human meningeal carcinomatosis (MS) tissue sections labeling Ephrin B2 with ab131536 at 1/200. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. the tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. A HRP-conjugated Goat Anti-Rabbit antibody was used as the secondary.
-
Western blot - Anti-Ephrin B2 antibody (ab131536)All lanes : Anti-Ephrin B2 antibody (ab131536) at 1/1000 dilution
Lane 1 : Rat spleen tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Peptide blocked mouse liver tissue lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody (ab131536)Immunocytochemistry/Immunofluorescence analysis of TM 3 cells labelling Ephrin B2 with ab131536 at a 1/75 dilution. A Cy3-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/50 dilution.
