Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
![Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)](https://www.abcam.com/ps/products/75/ab75867/Images/ab75867-258785-anti-p53-phospho-s33-antibody-ep2393y-western-blot.jpg "Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2393Y] to p53 (phospho S33)
- Suitable for: Dot blot, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho S33) antibody [EP2393Y]
See all p53 primary antibodies -
Description
Rabbit monoclonal [EP2393Y] to p53 (phospho S33) -
Host species
Rabbit -
Specificity
ab75867 detects p53 phosphorlyated at serine 33. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: T47D whole cell lysate treated with Etoposide. ICC/IF: A431 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2393Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)All lanes : Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867) at 1/5000 dilution
Lane 1 : T47D whole cell lysate - untreated
Lane 2 : T47D whole cell lysate - treated with Etoposide
Lane 3 : T47D whole cell lysate - treated with Etoposide followed by Alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)](https://www.abcam.com/ps/products/75/ab75867/Images/ab75867-259919-anti-p53-phospho-s33-antibody-ep2393y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab75867 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000).
Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP.
ab179477 (1/500) was used as a Pan control for ab75867. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)](https://www.abcam.com/ps/products/75/ab75867/Images/ab75867-91007-anti-p53-phospho-s33-antibody-ep2393y-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)ab75867 at 1/100 dilution staining phosphorylated p53 in A431 cells by Immunofluorescence. -
![Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)](https://www.abcam.com/ps/products/75/ab75867/Images/ab75867-255248-anti-p53-phospho-s33-antibody-ep2393y-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)Dot blot analysis of p53 (phospho S33) peptide (Lane 1) and p53 non-phospho peptide (Lane 2) labelling p53 (phospho S33) with ab75867 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)](https://www.abcam.com/ps/products/75/ab75867/Images/ab75867-6-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
