Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2393Y] to p53 (phospho S33)
  • Suitable for: Dot blot, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho S33) antibody [EP2393Y]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EP2393Y] to p53 (phospho S33)

  • Host species

    Rabbit

  • Specificity

    ab75867 detects p53 phosphorlyated at serine 33.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D whole cell lysate treated with Etoposide. ICC/IF: A431 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    Western blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    All lanes : Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867) at 1/5000 dilution

    Lane 1 : T47D whole cell lysate - untreated
    Lane 2 : T47D whole cell lysate - treated with Etoposide
    Lane 3 : T47D whole cell lysate - treated with Etoposide followed by Alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 1 minute


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)

    Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab75867 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000).

    Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP.

    ab179477 (1/500) was used as a Pan control for ab75867. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    ab75867 at 1/100 dilution staining phosphorylated p53 in A431 cells by Immunofluorescence.
  • Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    Dot Blot - Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)

    Dot blot analysis of p53 (phospho S33) peptide (Lane 1) and p53 non-phospho peptide (Lane 2) labelling p53 (phospho S33) with ab75867 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)
    Anti-p53 (phospho S33) antibody [EP2393Y] (ab75867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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