Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281906)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Armenian hamster monoclonal [HMHV-1B18] to TNFRSF14/HVEM - Chimeric
  • Suitable for: WB, ICC
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric
    See all TNFRSF14/HVEM primary antibodies

  • Description

    Armenian hamster monoclonal [HMHV-1B18] to TNFRSF14/HVEM - Chimeric

  • Host species

    Armenian hamster

  • Tested applications

    Suitable for: WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to Mouse TNFRSF14/HVEM. Raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein
    Database link: Q71F55

  • Positive control

    • ICC: RAW264.7 cells. WB: Mouse spleen, thymus and lung tissue lysates

  • General notes

    This chimeric hamster (Armenian) antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281906)
    Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281906)
    All lanes : Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281907) at 0.01 µg/ml

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse thymus tissue lysate
    Lane 3 : Mouse lung tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Anti-rabbit secondary antibody

    Predicted band size: 30 kDa



    This data was developed using the rabbit chimeric version (ab281907).

  • Immunocytochemistry - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281906)
    Immunocytochemistry - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281906)

    This data was developed using the rabbit chimeric version (ab281907).

    Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling TNFRSF14/HVEM with ab281907 at 10 µg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. RAW264.7 cells were fixed Shi-fix™ coverslips.

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