Anti-p53 DINP1/TP53INP1 antibody (ab125960)
Key features and details
- Rabbit polyclonal to p53 DINP1/TP53INP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p53 DINP1/TP53INP1 antibody
See all p53 DINP1/TP53INP1 primary antibodies -
Description
Rabbit polyclonal to p53 DINP1/TP53INP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human p53 DINP1/TP53INP1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab140403) -
Positive control
- This antibody gave a positive signal in Human Lung tissue lysate.
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General notes
This product was previously labelled as p53 DINP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Target
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Function
In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. -
Tissue specificity
Ubiquitously expressed. -
Cellular localization
Nucleus > PML body. - Information by UniProt
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Database links
- Entrez Gene: 94241 Human
- Omim: 606185 Human
- SwissProt: Q96A56 Human
- Unigene: 492261 Human
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Alternative names
- DKFZp434M1317 antibody
- FLJ22139 antibody
- p53 damage inducible nuclear protein 1 antibody
see all
Images
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Western blot - Anti-p53 DINP1/TP53INP1 antibody (ab125960)Anti-p53 DINP1/TP53INP1 antibody (ab125960) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125960 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab125960 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-p53 DINP1/TP53INP1 antibody (ab125960)Anti-p53 DINP1/TP53INP1 antibody (ab125960) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125960 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
