Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2849Y] to SIRT1 (phospho S47) - BSA and Azide free
  • Suitable for: ICC, Dot blot, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free
    See all SIRT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2849Y] to SIRT1 (phospho S47) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects SIRT1 phosphorylated on serine 47.

  • Tested applications

    Suitable for: ICC, Dot blot, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human gastric adenocarcinoma tissue

  • General notes

    ab247432 is the carrier-free version of ab76039 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247432 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/2000 dilution

    Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 treated with 1mM AICAR for 48 hours whole cell lysate
    Lane 3 : A549 treated with 1mM AICAR for 48 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 82 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76039, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)

    This data was developed using the same antibody clone in a different buffer formulation (ab76039).
    Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue sections labeling SIRT1 with purified ab76039 at 1/300 dilution. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
    Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) .
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument

  • Immunocytochemistry - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Immunocytochemistry - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    This data was developed using ab76039, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of A549 +/- LP cells labelling SIRT1 (phospho S47) with ab76039 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue).
  • Dot Blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Dot Blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    This data was developed using ab76039, the same antibody clone in a different buffer formulation.Dot blot analysis of SIRT1 (phospho S47) phospho peptide (Lane 1) and SIRT1 non-phospho peptide (Lane 2) labelling SIRT1 (phospho S47) with ab76039 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
  • Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/1000 dilution

    Lane 1 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : NIH:OVCAR-3 treated with 2mM H2O2 for 30 minutes whole cell lysate
    Lane 3 : NIH:OVCAR-3 treated with 2mM H2O2 for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 82 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    This data was developed using ab76039, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Western blot - Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/2000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate treated with LP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    This data was developed using ab76039, the same antibody clone in a different buffer formulation.

     

  • Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)
    Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - BSA and Azide free (ab247432)

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