Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor - BSA and Azide free
  • Suitable for: WB, ICC
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free
    See all Aryl hydrocarbon Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate; HeLa whole cell lysate.

  • General notes

    Ab232331 is the carrier-free version of ab190797. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232331 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)
    Immunocytochemistry - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(ab150077) was used as a secondary antibody at 1/1000 dilution.

    Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

    Counterstained with DAPI (blue). Negative controls used PBS instead of ab190797, then add ab150077 as the secondary antibody.

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)
    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 µg)
    Lane 4: HeLa whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. Ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190797).

  • Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)
    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] - BSA and Azide free (ab232331)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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