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Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]
    See all Aryl hydrocarbon Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type HAP1 whole cell lysate. PC-3, HeLa, HEK-293 and Caco-2 cell lysate. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7119(N)(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: Aryl hydrocarbon Receptor (KO) whole cell lysate (40 µg)
    Lane 4: HeLa whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190797 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab190797 was shown to specifically react with Aryl hydrocarbon Receptor when Aryl hydrocarbon Receptor knockout samples were used. Wild-type and Aryl hydrocarbon Receptor knockout samples were subjected to SDS-PAGE. ab190797 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/10,000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    Immunocytochemistry - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells showing both nuclear and cytoplasmic staining of Aryl hydrocarbon Receptor with ab190797 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488)(ab150077) was used as a secondary antibody at 1/1000 dilution.

    Fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

    Counterstained with DAPI (blue). Negative controls used PBS instead of ab190797, then add ab150077 as the secondary antibody.

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/1000 dilution

    Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?

  • Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)
    Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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