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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Helix-Turn-Helix

Anti-RFX2 antibody (ab79241)

Anti-RFX2 antibody (ab79241)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RFX2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RFX2 antibody
    See all RFX2 primary antibodies
  • Description

    Rabbit polyclonal to RFX2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human RFX2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab89390)

  • Positive control

    • This antibody gave a positive signal in the HeLa nuclear whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Helix-Turn-Helix

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 80 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 80 kDa).

Target

  • Sequence similarities

    Belongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P48378 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5990 Human
    • Omim: 142765 Human
    • SwissProt: P48378 Human
    • Unigene: 465709 Human
    • Alternative names

      • DNA-binding protein RFX2 antibody
      • HLA class II regulatory factor RFX2 antibody
      • regulatory factor X 2 (influences HLA class II expression) antibody
      • Regulatory factor X 2 antibody
      • regulatory factor X2 antibody
      • RFX2 antibody
      • RFX2_HUMAN antibody
      • trans acting regulatory factor 2 antibody
      see all

    Images

    • Western blot - Anti-RFX2 antibody (ab79241)
      Western blot - Anti-RFX2 antibody (ab79241)
      Anti-RFX2 antibody (ab79241) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 80 kDa
      Observed band size: 82 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab79241? Please let us know so that we can cite the reference in this datasheet.

    ab79241 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-RFX2 antibody (ab79241)
      Western blot - Anti-RFX2 antibody (ab79241)
      Anti-RFX2 antibody (ab79241) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 80 kDa
      Observed band size: 82 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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