Anti-eIF5A antibody [4E10F6] (ab204939)
Key features and details
- Mouse monoclonal [4E10F6] to eIF5A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-eIF5A antibody [4E10F6]
See all eIF5A primary antibodies -
Description
Mouse monoclonal [4E10F6] to eIF5A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human eIF5A aa 1 to the C-terminus. (Purified from E.coli).
Database link: P63241 -
Positive control
- Recombinant Human eIF5A protein; eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4E10F6 -
Isotype
IgG1 -
Research areas
Images
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Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution + Recombinant Human eIF5A protein
Predicted band size: 16 kDaExpected MWt is 42.8 kDa.
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All lanes : Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 16 kDa
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Flow cytometric analysis of HeLa cells labeling eIF5A with ab204939 at 1/200 dilution (green), compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling eIF5A with ab204939 at 1/200 dilution with DAB staining.