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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF5A antibody [4E10F6] (ab204939)

Anti-eIF5A antibody [4E10F6] (ab204939)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4E10F6] to eIF5A
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-eIF5A antibody [4E10F6]
    See all eIF5A primary antibodies
  • Description

    Mouse monoclonal [4E10F6] to eIF5A
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human eIF5A aa 1 to the C-terminus. (Purified from E.coli).
    Database link: P63241

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Recombinant Human eIF5A protein; eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human colon cancer tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4E10F6
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Images

  • Western blot - Anti-eIF5A antibody [4E10F6] (ab204939)
    Western blot - Anti-eIF5A antibody [4E10F6] (ab204939)
    Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution + Recombinant Human eIF5A protein

    Predicted band size: 16 kDa



    Expected MWt is 42.8 kDa.

  • Western blot - Anti-eIF5A antibody [4E10F6] (ab204939)
    Western blot - Anti-eIF5A antibody [4E10F6] (ab204939)
    All lanes : Anti-eIF5A antibody [4E10F6] (ab204939) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : eIF5A (aa1-154)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 16 kDa

  • Flow Cytometry - Anti-eIF5A antibody [4E10F6] (ab204939)
    Flow Cytometry - Anti-eIF5A antibody [4E10F6] (ab204939)

    Flow cytometric analysis of HeLa cells labeling eIF5A with ab204939 at 1/200 dilution (green), compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [4E10F6] (ab204939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [4E10F6] (ab204939)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling eIF5A with ab204939 at 1/200 dilution with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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