Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF5A antibody [EP526Y] (ab32443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP526Y] to eIF5A
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-eIF5A antibody [EP526Y]
    See all eIF5A primary antibodies

  • Description

    Rabbit monoclonal [EP526Y] to eIF5A

  • Host species

    Rabbit

  • Specificity

    This antibody shows low affinity in recognizing eIF5A2 recombinant protein.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human eIF5A aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P63241

  • Positive control

    • Jurkat and HeLa whole cell lysate (ab150035) and Adenocarcinoma of human uterus slides.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF5A antibody [EP526Y] (ab32443)
    Western blot - Anti-eIF5A antibody [EP526Y] (ab32443)
    Anti-eIF5A antibody [EP526Y] (ab32443) at 1/10000 dilution + Jurkat cell lysate.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody [EP526Y] (ab32443)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody [EP526Y] (ab32443)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eIF5A with purified ab32443 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [EP526Y] (ab32443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [EP526Y] (ab32443)

    ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-eIF5A antibody [EP526Y] (ab32443)
    Western blot - Anti-eIF5A antibody [EP526Y] (ab32443)
    All lanes : Anti-eIF5A antibody [EP526Y] (ab32443) at 1/2000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 18 kDa
    Observed band size: 15,20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking buffer: 5% NFDM/TBST

    This antibody shows low affinity in recognizing eIF5A2 recombinant protein.
  • Flow Cytometry - Anti-eIF5A antibody [EP526Y] (ab32443)
    Flow Cytometry - Anti-eIF5A antibody [EP526Y] (ab32443)
    Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32443, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Anti-eIF5A antibody [EP526Y] (ab32443)
    Anti-eIF5A antibody [EP526Y] (ab32443)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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