Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP526Y] to eIF5A - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster

Overview

  • Product name

    Anti-eIF5A antibody [EP526Y] - BSA and Azide free
    See all eIF5A primary antibodies

  • Description

    Rabbit monoclonal [EP526Y] to eIF5A - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody shows low affinity in recognizing eIF5A2 recombinant protein.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239816 is the carrier-free version of ab32443. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239816 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eIF5A with purified ab32443 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32443).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)

    ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    Flow Cytometry - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)

    This data was developed using ab32443, the same antibody clone in a different buffer formulation.

    Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32443, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Western blot - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    Western blot - Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    All lanes : Anti-eIF5A antibody [EP526Y] (ab32443) at 1/2000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 18 kDa
    Observed band size: 15,20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    This data was developed using ab32443, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST

    This antibody shows low affinity in recognizing eIF5A2 recombinant protein.
  • Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)
    Anti-eIF5A antibody [EP526Y] - BSA and Azide free (ab239816)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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