Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PERP antibody [EPR7885] (ab129083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7885] to PERP
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PERP antibody [EPR7885]
    See all PERP primary antibodies

  • Description

    Rabbit monoclonal [EPR7885] to PERP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues at the C terminus of Human PERP (UniProt ID: Q96FX8).

  • Positive control

    • WB: A431, HaCaT and HeLa cell lysates; Mouse heart, rat heart and human skin lysates. IHC-P: A431 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PERP antibody [EPR7885] (ab129083)
    Western blot - Anti-PERP antibody [EPR7885] (ab129083)
    All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PERP knockout HeLa cell lysate
    Lane 3 : HaCaT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab129083 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab129083 Anti-PERP antibody [EPR7885] was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265335 (knockout cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)
    Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)
    ab129083, at a dilution of 1/100, staining PERP in A431 cells by Immunofluorescence.
  • Western blot - Anti-PERP antibody [EPR7885] (ab129083)
    Western blot - Anti-PERP antibody [EPR7885] (ab129083)
    All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : A431 lysate
    Lane 4 : HACAT lysate
    Lane 5 : Human skin lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083)
    OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PERP antibody [EPR7885] (ab129083)
    Anti-PERP antibody [EPR7885] (ab129083)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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