Anti-PERP antibody (ab5986)
Key features and details
- Rabbit polyclonal to PERP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PERP antibody
See all PERP primary antibodies -
Description
Rabbit polyclonal to PERP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide:
EDDLLGNAKPRYFYTSA
, corresponding to amino acids 175-193 of Human PERP. -
General notes
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PERP antibody (ab5986)All lanes : Anti-PERP antibody (ab5986) at 1 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K569 cell lysate
Lane 6 : MCF-7 cell lysate
Lane 7 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
-
Western blot - Anti-PERP antibody (ab5986)Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1µ g /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody (ab5986)Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.
