Anti-SSH3 antibody (ab76945)
Key features and details
- Rabbit polyclonal to SSH3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SSH3 antibody
See all SSH3 primary antibodies -
Description
Rabbit polyclonal to SSH3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residues 609 and 659 of human SSH3 (NP_060327.2)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76945 was affinity purified using an epitope specific to SSH3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSH3 antibody (ab76945)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SSH3 with ab76945 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-SSH3 antibody (ab76945)All lanes : Anti-SSH3 antibody (ab76945) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-SSH3 antibody (ab76945)ab76945 at 1µg/ml staining SSH3 in HeLa whole cell lysate immunoprecipitated using ab76945 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
