Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)
![Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-2-ab252556343365antibetaActinantibodyBLR057FimmunocytochemistryHeLahuman.jpg "Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR057F] to beta Actin - BSA free
- Suitable for: IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-beta Actin antibody [BLR057F] - BSA free
See all beta Actin primary antibodies -
Description
Rabbit monoclonal [BLR057F] to beta Actin - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P Human
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Immunogen
Synthetic peptide within Human beta Actin aa 1-50. The exact sequence is proprietary. (NP_001092.1).
Database link: P60709 -
Positive control
- IHC-P: Human ovartian carcinoma tissue. ICC: HeLa cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
ab272085 is the carrier-free version of ab252556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR057F -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)
FFPE HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin using ab252556 at 1/200 dilution in ICC/IF, followed by HRP conjugated Goat Anti-Rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-1-ab252556343366antibetaActinantibodyBLR057Fimmunohistochemistryovariancarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for beta Actin using ab252556 at 1/400 dilution in immunohistochemical analysis, followed by HRP conjugated. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-3-benefits-of-recombinant-antibodies.png)
Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)
