Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18430] to Alpha Skeletal Muscle Actin
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430]
See all Alpha Skeletal Muscle Actin primary antibodies
Description

Rabbit monoclonal [EPR18430] to Alpha Skeletal Muscle Actin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat skeletal muscle and heart lysates; Human bladder lysate. IHC-P: Mouse and rat skeletal muscle tissues.
General notes
IHC is recommended for rat and mouse only.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18430
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in rat skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

All lanes : Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lane 9 : Human brain lysate
Lane 10 : Human heart lysate
Lane 11 : Human kidney lysate
Lane 12 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in mouse skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

All lanes : Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 5 seconds
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution + Rat skeletal muscle lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705) at 1/1000 dilution + Human bladder lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] (ab184705)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com