Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)

Key features and details

  • Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
  • Suitable for: WB, ICC
  • Reacts with: Rat, Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1]
    See all Alpha Skeletal Muscle Actin primary antibodies

  • Description

    Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin

  • Host species

    Mouse

  • Specificity

    In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Purified striated muscle actin from rabbit.

  • Positive control

    • WB: Whole extract of Rat heart ICC: HeLa cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Lane 1 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/500 dilution
    Lane 2 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/1000 dilution
    Lane 3 : Mouse Monoclonal Anti-Actin (a-Sarcomeric) antibody at 1/500 dilution

    All lanes : Whole extract of Rat heart

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 42 kDa



    chemiluminescent substrate.

  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) This image is courtesy of an Abreview submitted by Richard Patten.

    ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

    See Abreview

  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)
    Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) This image is courtesy of an Abreview submitted by Richard Patten.
    All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution

    All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)

    Secondary
    All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 second


    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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