Anti-ARPC1A antibody (ab133160)
Key features and details
- Goat polyclonal to ARPC1A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ARPC1A antibody
See all ARPC1A primary antibodies -
Description
Goat polyclonal to ARPC1A -
Host species
Goat -
Specificity
This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human ARPC1A aa 356-368 (C terminal) (Cysteine residue).
Sequence:C-DFKTLESSIQGLR
Database link: NP_006400.2 -
Positive control
- WB: HeLa cell lysate and mouse and rat brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARPC1A antibody (ab133160)All lanes : Anti-ARPC1A antibody (ab133160) at 0.1 µg/ml
Lane 1 : Mouse brain lysate (in RIPA buffer)
Lane 2 : Adult rat brain lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour.
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Western blot - Anti-ARPC1A antibody (ab133160)Anti-ARPC1A antibody (ab133160) at 0.3 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 42 kDaPrimary incubation for 1 hour.
