Anti-IFIT3/P60 antibody (ab236243)
Key features and details
- Rabbit polyclonal to IFIT3/P60
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IFIT3/P60 antibody
See all IFIT3/P60 primary antibodies -
Description
Rabbit polyclonal to IFIT3/P60 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant full length protein corresponding to Human IFIT3/P60 aa 1 to the C-terminus.
Database link: O14879 -
Positive control
- WB: A549 whole cell lysate. IHC-P: Human kidney and colon cancer tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-IFIT3/P60 antibody (ab236243) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
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Paraffin-embedded human kidney tissue stained for IFIT3/P60 using ab236243 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon cancer tissue stained for IFIT3/P60 using ab236243 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IFIT3/P60 (green) using ab236243 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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IFIT3/P60 was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236243 at 1/200 dilution.
Lane 1: Rabbit monoclonal IgG IP in HepG2 whole cell lysate.
Lane 2: ab236243 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.