Anti-IFIT3/P60 antibody (ab226375)
Key features and details
- Rabbit polyclonal to IFIT3/P60
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IFIT3/P60 antibody
See all IFIT3/P60 primary antibodies -
Description
Rabbit polyclonal to IFIT3/P60 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human -
Immunogen
Synthetic peptide within Human IFIT3/P60 aa 325-375. The exact sequence is proprietary.
Database link: O14879 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab226375 has not performed satisfactorily when used for WB of IFIT3/P60 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of IFIT3/P60.
Protein previously labeled as IFIT3 / P60.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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IFIT3/P60 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226375 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226375 at 0.4 µg/ml.
Lane 1: ab226375 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Cells prepared with NETN lysis buffer.