Antibodies, Proteins, Kits and Reagents for Life Science

Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

Key features and details

Mouse monoclonal [OTI1G1] to IFIT3/P60
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-IFIT3/P60 antibody [OTI1G1]
See all IFIT3/P60 primary antibodies
Description

Mouse monoclonal [OTI1G1] to IFIT3/P60
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human African green monkey
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human IFIT3/P60 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_001540).
Database link: O14879

Run BLAST with Run BLAST with
Positive control
- WB: HepG2, HeLa, A549, COS-7, Jurkat and MCF7 extracts; HEK-293T cells transfected with pCMV6-ENTRY IFIT3/P60 cDNA. ICC/IF: COS-7 (transiently transfected by pCMV6-ENTRY IFIT3/P60 cDNA) and HT-29 cells. Flow Cyt: Jurkat cells.
General notes

Clone OTI1G1 (formerly 1G1).

Protein previously labeled as IFIT3.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI1G1
Isotype

IgG1
Research areas

Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : MDCK (Canine kidney cell line) extracts
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) extracts

Lysates/proteins at 35 µg per lane.

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
Western blot - Anti-IFIT3/P60 antibody [OTI1G1] (ab118045)

All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 56 kDa

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