Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFITM1 antibody [EPR22620-12] (ab233545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22620-12] to IFITM1
  • Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IFITM1 antibody [EPR22620-12]
    See all IFITM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22620-12] to IFITM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, TF-1 and HeLa whole cell lysates. IP: K562 whole cell lysate. IHC-P: Human cerebrum and tonsil tissue. ICC/IF: K562 cells. Flow cyt: K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Immunocytochemistry/ Immunofluorescence - Anti-IFITM1 antibody [EPR22620-12] (ab233545)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,  0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) or A549 (human lung carcinoma cell line) cells labeling IFITM1 with ab233545 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in K-562 cells.  Negative control: A549 (PMID: 25105503). The nuclear cpounter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Western blot - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    All lanes : Anti-IFITM1 antibody [EPR22620-12] (ab233545) at 1/2000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HeLa, treated with 10ng/ml hIFN-a for 16 hours, whole cell lysate
    Lane 5 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25105503) IFN-α induce the expression of IFITM1(PMID: 27852071).

    Negative control: A549 (PMID: 25105503).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] (ab233545)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IFITM1  with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab233545 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Flow Cytometry - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Flow Cytometry - Anti-IFITM1 antibody [EPR22620-12] (ab233545)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) (Right) or A549 (human lung carcinoma cell line) (Left) cells labeling IFITM1 with ab233545 at 1/500 (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: A549 (PMID: 25105503).

  • Immunoprecipitation - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Immunoprecipitation - Anti-IFITM1 antibody [EPR22620-12] (ab233545)

    IFITM1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab233545 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233545 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (Input).
    Lane 2: ab233545 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233545 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFITM1 antibody [EPR22620-12] (ab233545)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling IFITM1  with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human cerebrum (PMID: 24603679). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab233545 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Anti-IFITM1 antibody [EPR22620-12] (ab233545)
    Anti-IFITM1 antibody [EPR22620-12] (ab233545)

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