Antibodies, Proteins, Kits and Reagents for Life Science

Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Key features and details

Mouse monoclonal [OTI4A1] to IRF3 - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-IRF3 antibody [OTI4A1] - BSA and Azide free
See all IRF3 primary antibodies
Description

Mouse monoclonal [OTI4A1] to IRF3 - BSA and Azide free
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human IRF3 aa 27-169. NP_001184053 Produced in E.coli
Database link: Q14653
Positive control
- WB: IRF3 transfecredHEK-293T cell lysate. IHC-P: Human colon adenocarcinoma, ovary, pancreas, thyroid and endometrium tissue.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.3
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

OTI4A1
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Paraffin embedded human colon adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

All lanes : Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663) at 1/2000 dilution

Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IRF3

Lysates/proteins at 5 µg per lane.

Predicted band size: 47 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Paraffin embedded human ovary tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Paraffin embedded human pancreas tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Paraffin embedded carcinoma of Human thyroid tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Paraffin embedded human endometrium adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com