violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)
![violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)](https://www.abcam.com/ps/products/253/ab253083/Images/ab253083-343656-antiinterferongammamonoclonalantibody-xmg12-flowcytometry-mouse.jpg "violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)")
Key features and details
- violetFluor™ 450 Rat monoclonal [XMG1.2] to Interferon gamma
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
- Isotype: IgG1
Overview
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Product name
violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2]
See all Interferon gamma primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [XMG1.2] to Interferon gamma -
Host species
Rat -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Mouse C57Bl/6 splenocytes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
XMG1.2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)
Flow cytometric analysis of mouse C57Bl/6 splenocytes (stimulated with PMA and Ionomycin) stained for Interferon gamma using ab253083 at 0.125 μg per reaction (Right) or a rat IgG1 violetFluor™ 450 isotype control (Left).
Cells were first stained with an Anti-Mouse CD4 FITC conjugated antibody before intracellular staining for Interferon gamma with ab253083.