Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP145] to Villin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), mIHC, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Villin antibody [SP145] - BSA and Azide free
    See all Villin primary antibodies

  • Description

    Rabbit monoclonal [SP145] to Villin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), mIHC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human colon, small intestine and kidney tissues; HT-29 cell lysate. mIHC: Human colon tissue.

  • General notes

    ab245749 is the carrier-free version of ab130751.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

    ab130751 staining Villin in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH 6. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG monoclonal (1/250) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab130751).

  • Flow Cytometry - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)
    Flow Cytometry - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

    Overlay histogram showing Caco 2 cells stained with ab130751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130751, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab130751).

  • Immunoprecipitation - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)
    Immunoprecipitation - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

    Villin was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Villin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab130751.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 93kDa; Villin

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab130751).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

    ab130751, at 1/100 dilution, staining Villin in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab130751).

  • Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)
    Anti-Villin antibody [SP145] - BSA and Azide free (ab245749)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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