Anti-Villin antibody (ab244291)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Villin aa 700 to the C-terminus.
Database link: P09327 -
Positive control
- IHC-P: Human kidney, small intestine, pancreas, colon, gallbladder and liver tissues. WB: Caco-2 cell lysate. ICC/IF: Caco-2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)
Paraffin-embedded human small intestine tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (ab244291)PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for Villin (green) using ab244291 at 4 µg/ml in ICC/IF.
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Western blot - Anti-Villin antibody (ab244291)All lanes : Anti-Villin antibody (ab244291) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 97 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human liver tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human gallbladder tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human colon tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human tonsil tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
Negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human pancreas tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab244291)Paraffin-embedded human kidney tissue stained for Villin using ab244291 at 1/50 dilution in immunohistochemical analysis.
