Anti-Villin antibody [EPR3490] (ab109516)
![Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-122430-anti-villin-antibody-epr3490-western-blot.jpg "Anti-Villin antibody [EPR3490] (ab109516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3490] to Villin
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Villin antibody [EPR3490]
See all Villin primary antibodies -
Description
Rabbit monoclonal [EPR3490] to Villin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3490 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Villin antibody [EPR3490] (ab109516)All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : HT-29 lysate
Lane 3 : Caco-2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-122429-anti-villin-antibody-epr3490-flow-cytometry.jpg)
Flow Cytometry - Anti-Villin antibody [EPR3490] (ab109516)Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green). -
![Anti-Villin antibody [EPR3490] (ab109516)](https://www.abcam.com/ps/products/109/ab109516/Images/ab109516-3-benefits-of-recombinant-antibodies.png)
Anti-Villin antibody [EPR3490] (ab109516)
