Anti-Villin antibody [5E3B2] (ab202021)
![Anti-Villin antibody [5E3B2] (ab202021)](https://www.abcam.com/ps/products/202/ab202021/Images/ab202021-243347-anti-villin-antibody-5e3b2-western-blot.jpg "Anti-Villin antibody [5E3B2] (ab202021)")
Key features and details
- Mouse monoclonal [5E3B2] to Villin
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-Villin antibody [5E3B2]
See all Villin primary antibodies -
Description
Mouse monoclonal [5E3B2] to Villin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Villin aa 1-250. Expressed in E. Coli.
Database link: P09327 -
Positive control
- Recombinant fragment of Villin (aa 1-209); Villin (aa: 1-209)-hIgGFc transfected HEK293 cell lysate; Human muscle and stomach cancer tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5E3B2 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Villin antibody [5E3B2] (ab202021)Anti-Villin antibody [5E3B2] (ab202021) at 1/500 dilution + Recombinant fragment of Villin (aa 1-209) at 1 µg
Predicted band size: 93 kDaExpected MW is 49 kDa
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![Western blot - Anti-Villin antibody [5E3B2] (ab202021)](https://www.abcam.com/ps/products/202/ab202021/Images/ab202021-243346-anti-villin-antibody-5e3b2-western-blot.jpg)
Western blot - Anti-Villin antibody [5E3B2] (ab202021)All lanes : Anti-Villin antibody [5E3B2] (ab202021) at 1/500 dilution
Lane 1 : Non transfected HEK293 cell lysate
Lane 2 : Villin (aa: 1-209)-hIgGFc transfected HEK293 cell lysate
Lysates/proteins at 1 µg per lane.
Predicted band size: 93 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (ab202021)](https://www.abcam.com/ps/products/202/ab202021/Images/ab202021-243345-anti-villin-antibody-5e3b2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (ab202021)Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling Villin using ab202021 at 1/200 dilution, followed by DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (ab202021)](https://www.abcam.com/ps/products/202/ab202021/Images/ab202021-243344-anti-villin-antibody-5e3b2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [5E3B2] (ab202021)Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling Villin using ab202021 at 1/200 dilution, followed by DAB staining.
