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Anti-Villin antibody [3E5G11] - N-terminal (ab201989)

Key features and details

  • Mouse monoclonal [3E5G11] to Villin - N-terminal
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Villin antibody [3E5G11] - N-terminal
    See all Villin primary antibodies

  • Description

    Mouse monoclonal [3E5G11] to Villin - N-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Villin aa 1-250 (N terminal).
    Database link: P09327

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Villin (AA: 1-209) recombinant protein; Villin (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate; SW620 cell lysate; HeLa and SW620 cells; Human cervical cancer tissue

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR286745-12 are from Tissue Culture Supernatant

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Villin with ab201989 at 1/200 dilution.

  • Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Flow Cytometry - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)

    Flow cytometric analysis of SW620 cells using ab201989 at 1/200 dilution (green) and negative control (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)

    Immunofluorescence analysis of Hela cells using ab201989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution + SW620 cell lysate

    Predicted band size: 93 kDa

  • Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    All lanes : Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Villin (AA: 1-209)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 93 kDa

  • Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Western blot - Anti-Villin antibody [3E5G11] - N-terminal (ab201989)
    Anti-Villin antibody [3E5G11] - N-terminal (ab201989) at 1/500 dilution + Human Villin (AA: 1-209) recombinant protein

    Predicted band size: 93 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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