Anti-Villin antibody (ab97512)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 7-256 of Human Villin (NP_009058).
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Positive control
- WB: HepG2 cell lysate, mouse and rat kidney extract. IHC-P: A549 xenograft tissue, mouse duodenum and kidney, rat colon, kidney. IF: HeLa whole cell.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Villin antibody (ab97512)Anti-Villin antibody (ab97512) at 1/2000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded A549 xenograft tissue stained Villin using ab97512 at a 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (ab97512)Confocal immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Villin with ab97512 (green) at 1/500 dilution. Red: Alpha-tubulin filaments, alpha Tubulin antibody at 1/2000.
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Western blot - Anti-Villin antibody (ab97512)Anti-Villin antibody (ab97512) at 1/1000 dilution + Mouse kidney tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Western blot - Anti-Villin antibody (ab97512)Anti-Villin antibody (ab97512) at 1/2000 dilution + Rat kidney tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded rat kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded mouse kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab97512)Paraffin-embedded rat colon tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
