Human Fetuin B ELISA Kit (ab240684)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.01 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Fetuin B ELISA Kit
See all Fetuin B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 8 3.9% Inter-assay Sample n Mean SD CV% plasma 3 4.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12.01 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 89% - 107% Serum 94 92% - 98% Hep Plasma 98 95% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fetuin B ELISA Kit (ab240684) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fetuin B protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fetuin B with 12.01 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fetuin B Capture Antibody 1 x 600µl 10X Human Fetuin B Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Fetuin B Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Tissue specificity
Liver and testis. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-B-type domains. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- 16G2
- 2310011O17Rik
- AI255764
see all -
Database links
- Entrez Gene: 26998 Human
- Omim: 605954 Human
- SwissProt: Q9UGM5 Human
- Unigene: 81073 Human
Images
-
Other - Human Fetuin B ELISA Kit (ab240684)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human Fetuin B standard curve in Sample Diluent NS.The Fetuin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Fetuin B in Human serum and plasma (heparin) samples.The concentrations of Fetuin B were measured in duplicates, interpolated from the Fetuin B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500 and plasma (heparin) 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin B concentration was determined to be 1237.10 ng/mL in serum and 1129.65 ng/mL in plasma (heparin). -
Serum from ten individual healthy Human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin B concentration was determined to be 1791.83 ng/mL with a range of 649.80 - 2979.58 ng/mL.
-
Sandwich ELISA - Human Fetuin B ELISA Kit (ab240684)To learn more about the advantages of recombinant antibodies see here.
