Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Fetuin B ELISA Kit (ab240684)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 12.01 pg/ml
  • Range: 93.75 pg/ml - 6000 pg/ml
  • Sample type: Cell culture supernatant, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Fetuin B ELISA Kit
    See all Fetuin B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    plasma 8 3.9%
    Inter-assay
    Sample n Mean SD CV%
    plasma 3 4.2%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    12.01 pg/ml

  • Range

    93.75 pg/ml - 6000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97 89% - 107%
    Serum 94 92% - 98%
    Hep Plasma 98 95% - 101%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Fetuin B ELISA Kit (ab240684) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fetuin B protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fetuin B with 12.01 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Fetuin B Capture Antibody 1 x 600µl
    10X Human Fetuin B Detector Antibody 2 vials
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Fetuin B Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Tissue specificity

    Liver and testis.

  • Sequence similarities

    Belongs to the fetuin family.
    Contains 2 cystatin fetuin-B-type domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • 16G2
    • 2310011O17Rik
    • AI255764
    • D17980
    • Fet
    • Fetub
    • FETUB_HUMAN
    • Fetuin beta
    • Fetuin-B
    • Fetuin-like protein
    • Fetuin-like protein IRL685
    • Gugu
    • IRL685
    • Pp63
    see all

  • Database links

    Images

    • Other - Human Fetuin B ELISA Kit (ab240684)
      Other - Human Fetuin B ELISA Kit (ab240684)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Human Fetuin B standard curve in Sample Diluent NS.
      Example of Human Fetuin B standard curve in Sample Diluent NS.
      The Fetuin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native Fetuin B in Human serum and plasma (heparin) samples.
      Interpolated concentrations of native Fetuin B in Human serum and plasma (heparin) samples.
      The concentrations of Fetuin B were measured in duplicates, interpolated from the Fetuin B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500 and plasma (heparin) 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin B concentration was determined to be 1237.10 ng/mL in serum and 1129.65 ng/mL in plasma (heparin).
    • Serum from ten individual healthy Human male donors was measured in duplicate.
      Serum from ten individual healthy Human male donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fetuin B concentration was determined to be 1791.83 ng/mL with a range of 649.80 - 2979.58 ng/mL.

    • Sandwich ELISA - Human Fetuin B ELISA Kit (ab240684)
      Sandwich ELISA - Human Fetuin B ELISA Kit (ab240684)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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