Biotin Anti-Transferrin antibody (ab34673)
Key features and details
- Biotin Rabbit polyclonal to Transferrin
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Transferrin antibody
See all Transferrin primary antibodies -
Description
Biotin Rabbit polyclonal to Transferrin -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Transferrin from Human serum.
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General notes
10-20 Biotin molecules per rabbit IgG molecule.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-Transferrin antibody (ab34673)
Multiplex western blot analysis of 35 µg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.
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Western blot - Biotin Anti-Transferrin antibody (ab34673)Biotin Anti-Transferrin antibody (ab34673) at 1/1000 dilution + Human depleted serum with Blocking buffer
Secondary
Peroxidase streptavidin conjugate at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa
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Western blot - Biotin Anti-Transferrin antibody (ab34673)Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.
