Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2932(2)] to Transferrin - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free
    See all Transferrin primary antibodies

  • Description

    Rabbit monoclonal [EPR2932(2)] to Transferrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma lysates; IHC-P: human liver tissue; Flow Cyt: HepG2 cells.

  • General notes

    ab239980 is the carrier-free version of ab109503 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239980 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Transferrin with Purified ab109503 at 1:300 dilution (2.19 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109503)
  • Flow Cytometry - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)
    Flow Cytometry - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)

    This data was developed using ab109503, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab109503 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)

    Immunohistochemical analysis of Transferrin expression in formalin-fixed, paraffin-embedded Human liver tissue, using unpurified ab109503 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109503).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)
    Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Transferrin antibody [EPR2932(2)] - BSA and Azide free (ab239980)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.