Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13115] to ARH - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARH antibody [EPR13115] - BSA and Azide free
    See all ARH primary antibodies

  • Description

    Rabbit monoclonal [EPR13115] to ARH - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250304 is the carrier-free version of ab181043. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250304 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab181043, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Immunoprecipitation - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)

    This data was developed using ab181043, the same antibody clone in a different buffer formulation.

    Western blot analysis of ARH in immunoprecipitation pellets from K562. ab181043 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1000 µg

    Predicted band size: 34 kDa



    This data was developed using ab181043, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Western blot - Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysatae

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    This data was developed using ab181043, the same antibody clone in a different buffer formulation.

     

  • Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)
    Anti-ARH antibody [EPR13115] - BSA and Azide free (ab250304)

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