Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control
    See all Transferrin primary antibodies

  • Description

    Rabbit monoclonal [EPR2932(2)] to Transferrin - Serum Loading Control

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma lysates; IHC-P: human liver tissue; Flow Cyt: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Transferrin with Purified ab109503 at 1:300 dilution (2.19 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • Flow Cytometry - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Flow Cytometry - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab109503 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    All lanes : Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/1000 dilution (unpurified)

    Lane 1 : fetal liver lysate
    Lane 2 : prostate cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 77 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)

    Immunohistochemical analysis of Transferrin expression in formalin-fixed, paraffin-embedded Human liver tissue, using unpurified ab109503 at 1/250 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)
    Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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