Human Factor XIII ELISA Kit (ab108836)
Key features and details
- Sensitivity: 2.1 ng/ml
- Range: 5 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor XIII ELISA Kit
See all Factor XIIIa kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% Overall 8.7% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 2.1 ng/ml -
Range
5 ng/ml - 80 ng/ml -
Recovery
99 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Factor XIII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of factor XIII in plasma, serum, milk, urine, saliva, and cell culture supernatants.
A Factor XIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XIII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XIII Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Factor XIII Microplate (12 x 8 well strips) 1 unit Factor XIII Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
Involvement in disease
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsThe activation peptide is released by thrombin. -
Cellular localization
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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Alternative names
- bA525O21.1 (coagulation factor XIII, A1 polypeptide)
- Coagulation factor XIII A chain
- Coagulation factor XIII A1 polypeptide
see all -
Database links
- Entrez Gene: 2162 Human
- Omim: 134570 Human
- SwissProt: P00488 Human
- Unigene: 335513 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108836