Rat PDGF BB ELISA Kit (ab267585)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat PDGF BB ELISA Kit
See all PDGF B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
8.19 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120.7 115% - 127% Plasma 100 93% - 108% Cell culture media 111.5 101% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat PDGF BB ELISA Kit is designed for the quantitative determination of PDGF BB in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for rat PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects rat and human PDGF BB.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti-Rat Rat PDGF BB coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rat Rat PDGF BB Detection Antibody 2 vials Rat Rat PDGF BB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 24628 Rat
- SwissProt: Q05028 Rat
- Unigene: 198230 Rat
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
