Mouse beta IG H3 ELISA Kit (ab206987)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 40 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse beta IG H3 ELISA Kit
See all IGH3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% EDTA plasma 8 4.4% Inter-assay Sample n Mean SD CV% EDTA plasma 3 4.9% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 107 103% - 109% Hep Plasma 107 105% - 110% EDTA Plasma 95 90% - 102% Cit plasma 89 83% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse beta IG H3 ELISA Kit (ab206987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta IG H3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse beta IG H3 with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse beta IG H3 (TGFBI) is a 683 amino acid (aa) protein with a 23 aa signal peptide, one EMI domain, four FAS1 domains, and one RGD motif. Mouse beta IG H3 is expressed as a 75 kDa protein with no post-translational additions. Following secretion, cleavages at multiple positions near the C-terminal end release peptides with pro-apoptotic activity. The four FAS1 domains of Mouse beta IG H3 contain YH motifs, which allow Mouse beta IG H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin. Mouse beta IG H3 is an adaptor protein that is induced in most cell types in response to TGF-beta stimulation. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts. Mouse beta IG H3 shares 91% aa sequence identity with human and porcine beta IG H3.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse beta IG H3 Capture Antibody (lyophilized) 1 vial Mouse beta IG H3 Detector Antibody (lyophilized) 1 vial Mouse beta IG H3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted, extracellular space, extracellular matrix. -
Alternative names
- Beta ig-h3
- beta-IG-H3
- BIGH3
see all -
Database links
- Entrez Gene: 21810 Mouse
- SwissProt: P82198 Mouse
Images
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Other - Mouse beta IG H3 ELISA Kit (ab206987)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of the mouse beta IG H3 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of the mouse beta IG H3 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution of mouse beta IG H3 in plasma samples.Native mouse beta IG H3 was measured in 0.8% mouse plasma citrate, 0.8% mouse plasma EDTA, and 0.33% mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Linearity of dilution of mouse beta IG H3 in cell culture media.Recombinant mouse beta IG H3 was spiked into 50% cell culture media without FBS and into 10% cell culture media with FBS and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Linearity of mouse beta IG H3 in mouse eyeball tissue extract.Native mouse beta IG H3 was measured in 100 μg/mL of mouse eyeball extract diluted in a 2-fold dilution series in Sample Diluent 1X Cell Extraction Buffer PTR. The concentrations of mouse beta IG H3 were measured in duplicate and interpolated from the mouse beta IG H3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Assay specificity is demonstrated on 3T3-L1 cells.3T3-L1 cells were cultured in cell culture media in the presence and absence 1 µM Dexamethasone, 0.5 mM IBMX, 1 µg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 1 mg/mL using this kit. The concentrations of beta IG H3 were measured in duplicate and interpolated from the beta IG H3 standard. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Sandwich ELISA - Mouse beta IG H3 ELISA Kit (ab206987)To learn more about the advantages of recombinant antibodies see here.
