Mouse beta IGH3 ELISA Kit (ab193717)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse beta IGH3 ELISA Kit
See all IGH3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
3 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 140.1 132% - 148% Plasma 136 115% - 150% Cell culture media 143.6 137% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s beta IGH3 mouse ELISA Kit (ab193717) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BIGH3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse beta IGH3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta IGH3 present in the samples. The wells are washed and biotinylated anti-mouse beta IGH3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta IGH3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated mouse beta-IGH3 detection antibody (lyophilized) 2 vials Mouse beta-IGH3 standards (lyophilized) 2 vials Pre-coated mouse beta-IGH3 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Cellular localization
Secreted, extracellular space, extracellular matrix. -
Alternative names
- Beta ig-h3
- beta-IG-H3
- BIGH3
see all -
Database links
- Entrez Gene: 21810 Mouse
- SwissProt: P82198 Mouse
Images
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Standard Curve.
Typical Standard Curve using ab193717 BIGH3 Mouse ELISA Kit.
