HRP Anti-Collagen VI antibody [EPR17072] (ab200596)
![HRP Anti-Collagen VI antibody [EPR17072] (ab200596)](https://www.abcam.com/ps/products/200/ab200596/Images/ab200596-246676-anti-collagen-vi-antibody-epr17072-hrp-western-blot.jpg "HRP Anti-Collagen VI antibody [EPR17072] (ab200596)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17072] to Collagen VI
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Collagen VI antibody [EPR17072]
See all Collagen VI primary antibodies -
Description
HRP Rabbit monoclonal [EPR17072] to Collagen VI -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human Collagen VI aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P12109 -
Positive control
- WB: Human skeletal muscle lysate and WI-38 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17072 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Collagen VI antibody [EPR17072] (ab200596)All lanes : HRP Anti-Collagen VI antibody [EPR17072] (ab200596) at 1/5000 dilution
Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 109 kDa
Observed band size: 147 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200596 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Collagen VI antibody [EPR17072] (ab200596)](https://www.abcam.com/ps/products/200/ab200596/Images/ab200596-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Collagen VI antibody [EPR17072] (ab200596)
