Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1373Y] to Apolipoprotein E - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free
    See all Apolipoprotein E primary antibodies

  • Description

    Rabbit monoclonal [EP1373Y] to Apolipoprotein E - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell and human liver cancer tissue lysates.

  • General notes

    ab171357 is the carrier-free version of ab51015 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab171357 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling Apolipoprotein with unpurified ab51015 at 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Immunocytochemistry - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Apolipoprotein E with purified ab51015 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
  • Flow Cytometry - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Flow Cytometry - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of 293T cells labelling Apolipoprotein E with purified ab51015 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution (purified) + Human serum at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Western blot - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Anti-Apolipoprotein E antibody [EP1373Y] (ab51015) at 1/10000 dilution (unpurified) + Human serum at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    This data was developed using ab51015, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Apolipoprotein E with purified ab51015 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Immunoprecipitation - Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

    This data was developed using ab51015, the same antibody clone in a different buffer formulation.

    ab51015 (purified) at 1/30 immunoprecipitating Apolipoprotein in human fetal liver tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)
    Anti-Apolipoprotein E antibody [EP1373Y] - BSA and Azide free (ab171357)

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