Anti-MCM4 antibody (ab4459)
Key features and details
- Rabbit polyclonal to MCM4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM4 antibody
See all MCM4 primary antibodies -
Description
Rabbit polyclonal to MCM4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - 32 amino acids which represented a portion of human MCM 4 encoded within exon 14.
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Positive control
- IHC-P: FFPE human testis tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM4 antibody (ab4459)HeLa cell RIPA extract incubated with 0.2
µ g/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-MCM4 antibody (ab4459)Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg
Secondary
Goat anti rabbit HRP. at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 96.6 kDa
This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image. -
Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
