Mouse PON2 Matched Antibody Pair Kit (ab216067)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
-
Product name
Mouse PON2 Matched Antibody Pair Kit
See all PON2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
19.1 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PON2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PON2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208984. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse PON2 Capture Antibody 2 x 50µg 1 x 50µg Mouse PON2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse PON2 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. -
Tissue specificity
Widely expressed with highest expression in liver, lung, placenta, testis and heart. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- A esterase 2
- A-esterase 2
- Aromatic esterase 2
see all -
Database links
- Entrez Gene: 330260 Mouse
- SwissProt: Q62086 Mouse
- Unigene: 126984 Mouse
- Unigene: 460999 Mouse
Images
-
Standard calibration curve. Background subtracted values are graphed.
-
To learn more about the advantages of recombinant antibodies see here.