Anti-MCM4 antibody (ab154315)
Key features and details
- Rabbit polyclonal to MCM4
- Suitable for: WB, IHC-P, ICC/IF, ChIP, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM4 antibody
See all MCM4 primary antibodies -
Description
Rabbit polyclonal to MCM4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, ChIP, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human MCM4 aa 595-835 (internal sequence).
Database link: P33991 -
Positive control
- WB: Jurkat, Raji, NCI-H929; Neuro2A, C8D30 , NIH/3T3 , Raw264.7 , C2C12 whole cell lysate. IHC-P: A549 xenograft tissue. ICC/IF: HeLa cells. IP: Jurkat whole cell extract. ChIP: HeLa cells.
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General notes
Keep as concentrated solution.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM4 antibody (ab154315)All lanes : Anti-MCM4 antibody (ab154315) at 1/5000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa7.5 % SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab154315)Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling MCM4 using ab154315 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab154315)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MCM4 using ab154315 at 1/500 a dilution. Right panel costained with Hoechst 33342. -
Immunoprecipitation - Anti-MCM4 antibody (ab154315)MCM4 was immunoprecipitated from 40 μg of Jurkat whole cell lysate with ab154315 at 1/500 dilution. 7.5 % SDS-PAGE. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: Jurkat lysate 40 μg (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody. -
Western blot - Anti-MCM4 antibody (ab154315)All lanes : Anti-MCM4 antibody (ab154315) at 1/1000 dilution
Lane 1 : Neuro2A whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 whole cell extract
Lane 4 : Raw264.7 whole cell extract
Lane 5 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa7.5% SDS-PAGE
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ChIP - Anti-MCM4 antibody (ab154315)Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
