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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-MCM4 antibody (ab88546)

Anti-MCM4 antibody (ab88546)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to MCM4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MCM4 antibody
    See all MCM4 primary antibodies
  • Description

    Mouse polyclonal to MCM4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-863 of Human MCM4 (AAH31061.1)

  • Positive control

    • WB: placenta or HeLa cell lysate; IHC: tonsil tissue; ICC/IF: Hela cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Images

  • Western blot - Anti-MCM4 antibody (ab88546)
    Western blot - Anti-MCM4 antibody (ab88546)
    All lanes : Anti-MCM4 antibody (ab88546) at 1 µg/ml

    Lane 1 : transfected 293T cells
    Lane 2 : non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 96 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab88546)
    Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab88546)
    ab88546 at 10ug/ml staining MCM4 by immunofluorescence on HeLa cells.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab88546)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab88546)
    ab88546 at 3ug/ml staining MCM4 on formalin-fixed, paraffin-embedded Human tonsil tissue.
  • Western blot - Anti-MCM4 antibody (ab88546)
    Western blot - Anti-MCM4 antibody (ab88546)
    Anti-MCM4 antibody (ab88546) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size: 96 kDa

  • Western blot - Anti-MCM4 antibody (ab88546)
    Western blot - Anti-MCM4 antibody (ab88546)
    Anti-MCM4 antibody (ab88546) at 1 µg/ml + Human placenta lysate at 50 µg

    Predicted band size: 96 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MCM4 antibody (ab88546)

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    Anti-MCM4 (phospho S54) antibody (ab74014)

    Applications: WB

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    Anti-MCM4 antibody (ab4459)

    Applications: ICC/IF, IHC-P, WB

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    Anti-MCM4 antibody [EPR5245] (ab124836)

    Applications: Flow Cyt, WB

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    Anti-MCM4 antibody (ab154315)

    Applications: ChIP, ICC/IF, IHC-P, IP, WB

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    Anti-MCM4 antibody (ab84153)

    Applications: IHC-P

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    Anti-MCM4 antibody (ab3728)

    Applications: IHC-P, WB

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