Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742]
    See all KDM5A / Jarid1A / RBBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12742] to KDM5A / Jarid1A / RBBP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human KDM5A/ Jarid1A/ RBBP2 aa 1450-1550 (Cysteine residue). The exact sequence is proprietary.
    Database link: P29375

  • Positive control

    • HeLa, 293T, MCF7 and NCCIT cell lysates; permeabilized NCCIT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
    Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: KDM5A knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab177486 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab177486 was shown to specifically react with KDM5A in wild-type HAP1 cells. No bands were observed when KDM5A knockout samples were used. Wild-type and KDM5A knockout samples were subjected to SDS-PAGE. Ab177486 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
    Flow Cytometry - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)

    Flow Cytometric analysis of permeabilized NCCIT cells labeling KDM5A / Jarid1A / RBBP2 with ab177486 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
    Western blot - Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
    All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : NCCIT cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 192 kDa

  • Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)
    Anti-KDM5A / Jarid1A / RBBP2 antibody [EPR12742] (ab177486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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