Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Reductase antibody [EPR7238] (ab124995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7238] to Glutathione Reductase
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutathione Reductase antibody [EPR7238]
    See all Glutathione Reductase primary antibodies

  • Description

    Rabbit monoclonal [EPR7238] to Glutathione Reductase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Glutathione Reductase aa 500-600. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, A549 and Caco-2 cell lysates; permeabilized HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE.  Ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Flow Cytometry - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    ab124995, at a 1/10 dilution, staining Glutathione Reductase in permeabilized HeLa cells by Flow Cytometry (red). Analysis with a rabbit IgG (negative control) is shown in green.
  • Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

    Lane 1 : mouse brain
    Lane 2 : rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Western blot - Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse

    Predicted band size: 56 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Anti-Glutathione Reductase antibody [EPR7238] (ab124995)
    Anti-Glutathione Reductase antibody [EPR7238] (ab124995)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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